Table 1.

Summary statistics for pyrosequencing analysis of M. sexta ESTs

	06 a	CF	CH	IF	IH	CIFH b	06CIFH c
Total number of reads	95,458 (95,358)	227,302	647,587	405,739	541,024	1,821,652	1,917,110
Average reads length (bp)	185 (185)	296	287	293	287	289	284
Total number of contigs	1,471 (7,231)	2,118	11,540	4,063	10,600	19,020	19,504
Contigs size (avg./longest in bp)	391/3,552 (300/3,909)	770/12,740	827/11,667	764/8,482	832/10,591	923/23,095	911/23,097
Total assembled reads	64,874 (69,429)	191,156	561,054	349,028	465,561	1,677,738	1,757,333
Singlet reads	28,518 (25,929)	32,518	68,861	49,444	61,108	108,587	120,670
Singlet length (avg. in bp)	179	244	245	235	254	209	200
Total BLASTable sequences	29,989	34,636	80,401	53,507	71,708	127,607	140,174
Orphan sequences (no BLAST match, #/%)	19,963/67	17,982/52	51,968/65	28,649/54	46,521/65	73,915/58	89,948/64
Contigs and reads with functional assignment	10,026	16,654	28,433	24,858	25,187	53,692	50,226

^aResults from reanalysis of the 2006 sequence data. The numbers in parentheses (adopted from Zou et al., 2008) are listed for comparison with the new results.

^bAnalysis of the 2009 EST sequences of control fat body (CF), control hemocytes (CH), induced fat body (IF), and induced hemocytes (IH) from M. sexta larvae.

^cAnalysis of the combined reads of 2006 (raw flow signals interpreted with the up-graded software) and 2009 (CF, CH, IF, and IH).