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. Author manuscript; available in PMC: 2012 Sep 1.
Published in final edited form as: Insect Biochem Mol Biol. 2011 May 27;41(9):733–746. doi: 10.1016/j.ibmb.2011.05.005

Table 9.

A list of FB CIFH contigs with BLAST hits*

CIFH contig # Original read # RA or ARN BLAST results
CF CH IF IH Total CF/CH IF/IH
00051 291 1 329 0 621 1403.9 681.6 gi|183979376|dbj|BAG30740.1| muscle myosin heavy chain [Papilio xuthus]
00153 etc. 2069 4 2563 1 4637 2495.3 5309.8 gi|2498144|sp|Q25490.1 apoLp (00153 02405 02406 03748 04510 06831 06834 07770 14087 14589)
00194 37 0 81 1 119 178.5 167.8 gi|48476133|gb|AAT44358.1| calcium-activated potassium channel α subunit [Manduca sexta]
00285 & 298 23 921 5 1247 62.5 381.6 gi|73921301|gb|AAG42021.2|AF327882_1 JHE precursor [Manduca sexta] (00285, 00859)
00409 168 0 216 0 384 810.5 447.5 gi|110750043|ref|XP_394261.3| plexin A CG11081-PA, isoform A [Apis mellifera]
00414 58 1 50 0 109 279.8 103.6 gi|195382713|ref|XP_002050074.1| GJ21937 [Drosophila virilis]
00423 149 0 220 0 369 718.8 455.8 gi|158295580|ref|XP_316291.4| AGAP006225-PA [Anopheles gambiae]
00465 134 1 230 0 365 646.4 476.5 gi|149755131|ref|XP_001491560.1| hemicentin 1 [Equus caballus]
00535 67 1 100 0 168 323.2 207.2 gi|242015135|ref|XP_002428229.1| thrombospondin-3 precursor [Pediculus humanus corporis]
00575 3 0 259 5 267 14.5 107.3 gi|154240658|dbj|BAF74637.1| peptidoglycan recognition protein-D [Samia cynthiaricini]
00609 324 0 762 0 1086 1563.1 1578.6 gi|225542786|gb|ACN91276.1| dentin sialophosphoprotein precursor [Bos taurus]
00737 2 4 131 2 139 2.4 135.7 gi|198466442|ref|XP_002135189.1| GA23919 [Drosophila pseudoobscura]
00748 131 4 118 2 255 158.0 122.2 gi|29346557|ref|NP_810060.1| glycine dehydrogenase [Bacteroides thetaiotaomicron]
00766 45 0 74 1 120 217.1 153.3 gi|158293377|ref|XP_314728.3| AGAP008632-PA [Anopheles gambiae]
00773 49 12 93 1 155 19.7 192.7 gi|157103945|ref|XP_001648193.1| dihydropyrimidine dehydrogenase [Aedes aegypti]
00785 120 2 139 2 263 289.5 144.0 gi|193795848|gb|ACF21977.1| paramyosin [Bombyx mandarina]
00884 39 1 23 0 63 188.1 47.6 gi|156553304|ref|XP_001599652.1| GA21752-PA [Nasonia vitripennis]
00960 52 2 99 1 154 125.4 205.1 gi|157107996|ref|XP_001650030.1| sarcosine dehydrogenase [Aedes aegypti]
01095 64 0 99 1 164 308.8 205.1 gi|169639235|gb|ACA60733.1| venom acid phosphatase [Pteromalus puparum]
01097 134 2 436 5 577 323.2 180.7 gi|55139125|gb|AAV41236.1| immulectin-3 [Manduca sexta]
01127 41 1 52 1 95 197.8 107.7 gi|189491898|gb|ACE00761.1| adipokinetic hormone receptor [Manduca sexta]
01454 599 3 1337 3 1942 963.2 923.3 gi|91082539|ref|XP_973726.1| inter-α (globulin) inhibitor H4 (Kallikrein-sensitive) [T. castaneum]
01480 211 0 729 0 940 1017.9 1510.3 gi|183979392|dbj|BAG30748.1| hypothetical protein [Papilio xuthus]
01601 60 1 79 0 140 289.5 163.7 gi|270005801|gb|EFA02249.1| hypothetical protein TcasGA2 TC007912 [Tribolium castaneum]
01742 65 0 75 0 140 313.6 155.4 gi|283100192|gb|ADB08386.1| sugar transporter 4 [Bombyx mori]
01743 27 0 112 0 139 130.3 232.0 gi|134252572|gb|ABO65045.1| β-hexosaminidase [Ostrinia furnacalis]
01870 184 0 323 0 507 887.7 669.2 gi|242010783|ref|XP_002426138.1| conserved hypothetical protein [Pediculus humanus corporis]
01892 82 0 108 0 190 395.6 223.7 gi|158289807|ref|XP_311448.4| AGAP010734-PA [Anopheles gambiae]
01915 85 2 275 0 362 205.0 569.7 gi|110757936|ref|XP_623940.2| peroxidase precursor (DmPO) [Apis mellifera]
01956 127 0 99 0 226 612.7 205.1 gi|156551746|ref|XP_001602035.1| ENSANGP00000015052 [Nasonia vitripennis]
01972 etc. 383 0 3327 0 3710 1847.7 6892.5 gi|136206|sp|P22297.1|transferrin (01972 10382 11027 14937 17193 17206 17395 16606 18234 18308)
02101 51 0 75 0 126 246.0 155.4 gi|186909546|gb|ACC94296.1| glucose oxidase-like enzyme [Helicoverpa armigera]
02104 59 1 67 1 128 284.6 138.8 gi|91079628|ref|XP_967731.1| AGAP002355-PA [Tribolium castaneum]
02137 101 0 24 0 125 487.2 49.7 gi|91084191|ref|XP_967340.1| AGAP002557-PA [Tribolium castaneum]
02144 82 0 132 3 217 395.6 91.2 gi|62002223|gb|AAX58711.1| pheromone-degrading enzyme 1 [Antheraea polyphemus]
02166 60 0 57 0 117 289.5 118.1 gi|193876254|gb|ACF24761.1| lipid storage droplet protein 1 [Manduca sexta]
02184 53 2 111 1 167 127.8 230.0 gi|226342886|ref|NP_001139705.1| serpin 13 [Bombyx mori]
02219 454 3 971 3 1431 730.1 670.5 gi|219815604|gb|ACL36977.1| putative ecdysone oxidase [Helicoverpa zea]
02329 143 0 411 0 554 689.9 851.5 gi|112984054|ref|NP_001037422.1| yellow1 [Bombyx mori]
02337 & 107 2 170 7 286 258.1 50.3 gi|91079867|ref|XP_967070.1| AGAP005945-PB [Tribolium castaneum] (02337, 15796)
02361 7 4 70 1 82 8.4 145.0 gi|56418425|gb|AAV91020.1| hemolymph proteinase 22 [Manduca sexta]
02393 45 0 77 5 127 217.1 31.9 gi|156545523|ref|XP_001607196.1| dihydroxyacetone kinase-2 homolog (yeast) [Nasonia vitripennis]
02394 28 1 23 0 52 135.1 47.6 gi|91077746|ref|XP_966706.1| conserved hypothetical protein [Tribolium castaneum]
02409 113 0 187 0 300 545.1 387.4 gi|109502352|gb|ABE01157.2| carboxylesterase [Spodoptera litura]
02482 63 0 85 1 149 303.9 176.1 gi|66519258|ref|XP_625210.1| ~ CG6188-PA [Apis mellifera]
02609 97 0 146 2 245 468.0 151.2 gi|156968285|gb|ABU98614.1| α-amylase [Helicoverpa armigera]
02638 & 241 0 206 0 447 1162.6 426.8 gi|41016826|sp|Q27772.3|C1TC_SPOFR C-1-THF synthase, cytoplasmic (02638, 07658)
02651 24 0 124 0 148 115.8 256.9 gi|5326830|gb|AAD42058.1|AF122899_1 plasmatocyte-spreading peptide precursor [Manduca sexta]
02800 28 0 97 0 125 135.1 201.0 gi|260765449|gb|ACX49762.1| β-fructofuranosidase 1 [Manduca sexta]
02847 33 0 103 0 136 159.2 213.4 gi|114051702|ref|NP_001040423.1| zinc-containing alcohol dehydrogenase [Bombyx mori]
02931 & 187 0 429 0 616 902.1 888.8 gi|1658003|gb|AAB18243.1| microsomal epoxide hydrolase [Trichoplusia ni] (02931, 04388)
02947 518 21 981 56 1576 119.0 36.3 gi|259493819|gb|ACW82749.1| hemocyte aggregation inhibitor protein precursor [Manduca sexta]
02979 49 0 92 4 145 236.4 47.6 gi|52782757|sp|Q9NJ98.1|BGRP1_MANSE β-1,3-glucan recognitionprotein 1 βGRP-1
02985 3 0 158 0 161 14.5 327.3 gi|56418466|gb|AAV91027.1| serine proteinase-like protein 4 [Manduca sexta]
03185 106 0 234 10 350 511.4 48.5 gi|157117489|ref|XP_001658792.1| 3-hydroxyacyl-CoA dehyrogenase [Aedes aegypti]
03224 98 0 477 0 575 472.8 988.2 gi|226342906|ref|NP_001139715.1| serpin 22 [Bombyx mori]
03226 222 0 663 0 885 1071.0 1373.5 gi|153791757|ref|NP_001093275.1| myo-inositol oxygenase [Bombyx mori]
03395 22 1 24 0 47 106.1 49.7 gi|157908523|dbj|BAF81491.1| juvenile hormone epoxide hydrolase [Bombyx mori]
03415 190 0 216 1 407 916.6 447.5 gi|2708688|gb|AAB92583.1| acyl-CoA 9 desaturase [Trichoplusia ni]
03434 1 0 387 0 388 4.8 801.7 gi|189234566|ref|XP_001815977.1| Kaz1-ORFB CG1220-PE [Tribolium castaneum]
03454 28 0 102 0 130 135.1 211.3 gi|6560669|gb|AAF16712.1|AF117590_1 unknown [Manduca sexta]
03483 280 0 374 0 654 1350.8 774.8 gi|283558277|gb|ADB27116.1| aliphatic nitrilase [Bombyx mori]
03712 49 2 157 5 213 118.2 65.1 gi|170779021|gb|ACB36909.1| glutathione S-transferase θ [Antheraea pernyi]
03737 167 1 197 0 365 805.6 408.1 gi|56462300|gb|AAV91433.1| putative serine protease-like protein 2 [Lonomia obliqua]
03776 etc. 204 8 960 51 1223 123.0 39.0 gi|112983872|ref|NP_001036857.1| serpin-like protein [Bombyx mori] (03776, 06215, 06531, 17814)
04012 & 167 3 727 11 908 268.5 136.9 gi|27733411|gb|AAO21503.1|AF413062_1 leureptin, LPS binding [Manduca sexta] (04012, 08453)
04413 69 1 133 1 204 332.9 275.5 gi|194743582|ref|XP_001954279.1| GF18195 [Drosophila ananassae]
04424 72 0 64 0 136 347.3 132.6 gi|114052020|ref|NP_001040445.1| tropomyosin 1 [Bombyx mori]
04430 74 0 68 0 142 357.0 140.9 gi|114052573|ref|NP_001040481.1| phosphoribosyl pyrophosphate synthetase [Bombyx mori]
04498 46 0 115 0 161 221.9 238.2 gi|90025232|gb|ABD85119.1| JH epoxide hydrolase [Spodopteraexigua]
04504 53 0 135 0 188 255.7 279.7 gi|7239259|gb|AAF43151.1|AF226857_1 hemolymph JHBP precursor [Manduca sexta]
04722 & 578 0 861 0 1439 2788.4 1783.7 gi|116791778|gb|ABK26104.1| unknown [Picea sitchensis] (04722, 04994)
04781 56 0 237 0 293 270.2 491.0 gi|118359591|ref|XP_001013035.1| PHD-finger family protein [Tetrahymenathermophila]
04786 61 0 62 0 123 294.3 128.4 gi|219686082|emb|CAW30924.1| putative aldo-ketose reductase 1 [Papilio dardanus]
04791 144 0 200 0 344 694.7 414.3 gi|116788175|gb|ABK24783.1| unknown [Picea sitchensis]
04806 518 1 372 0 891 2499.0 770.7 gi|157122933|ref|XP_001659963.1| actin [Aedes aegypti]
04808 0 0 426 2 428 0.0 441.3 gi|237861314|gb|AAV41237.2| immulectin-4 [Manduca sexta]
04830 etc. 59 2 755 6 822 142.3 260.7 gi|169646838|ref|NP_001112375.1| heat shock protein 25.4 [Bombyx mori] (04830, 04887, 05717)
05038 & 101 0 175 1 277 487.2 362.5 gi|110759694|ref|XP_394781.3| rTS β protein [Apis mellifera] (05038, 05832)
05136 1074 11 1041 37 2163 471.0 58.3 gi|114051966|ref|NP_001040198.1| mitochondrial aldehyde dehydrogenase [Bombyx mori]
05324 68 0 88 0 156 328.0 182.3 gi|225346695|gb|ACN86370.1| troponin I transcript variant C [Bombyx mandarina]
05348 50 0 67 0 117 241.2 138.8 gi|189234391|ref|XP_974849.2| GA16498-PA [Tribolium castaneum]
05417 etc. 273 0 917 0 1190 1317.0 1899.7 gi|260907784|gb|ACX53694.1| alcohol DH [Heliothis virescens] (05417, 05461, 07389, 07432)
05984 89 0 97 0 186 429.4 201.0 gi|56462260|gb|AAV91413.1| myosin 3 light chain [Lonomia obliqua]
06175 11 0 52 1 64 53.1 107.7 gi|170070451|ref|XP_001869584.1| conserved hypothetical protein [Culex quinquefasciatus]
06227 251 1 715 0 967 1210.9 1481.3 gi|124527|sp|Q00630.1|ICYB_MANSE insecticyanin-B, blue biliprotein
06251 66 2 57 7 132 159.2 16.9 gi|158289206|ref|XP_310956.4| AGAP000179-PA [Anopheles gambiae]
06394 51 0 228 0 279 246.0 472.3 gi|110611262|gb|ABG77980.1| alanine-glyoxylate transaminase 1 [Glossinamorsitans morsitans]
06588 60 0 75 0 135 289.5 155.4 gi|56462256|gb|AAV91411.1| myosin 1 light chain [Lonomia obliqua]
06597 60 0 200 0 260 289.5 414.3 gi|56462320|gb|AAV91443.1| secreted peptide 30 [Lonomia obliqua]
06732 115 1 244 0 360 554.8 505.5 gi|25090512|sp|Q25513.1|HGLY_MANSE 27 kDa hemolymph glycoprotein
06789 & 159 0 460 0 619 767.1 953.0 gi|156968291|gb|ABU98617.1| unknown [Helicoverpa armigera] (06789, 06876)
06975 & 106 2 212 2 322 255.7 219.6 gi|189237651|ref|XP_001813448.1| N-acetyl neuraminatelyase [Tribolium castaneum] (06975, 14637)
07116 & 1 4 1015 4 1024 1.2 525.7 gi|171262319|gb|ACB45566.1| lebocin-like protein [Antheraea pernyi] (07116, 10853)
07565 24 1 14 0 39 115.8 29.0 gi|7862150|gb|AAF70499.1|AF255341_1 3-dehydroecdysone 3α-reductase [Spodoptera littoralis]
07608 etc. 353 3 3931 0 4287 567.7 8143.9 gi|159526|gb|AAA29320.1| Met-rich storage protein 1 (07608, 07975, 08141, 14688)
07629 65 0 82 0 147 313.6 169.9 gi|77415676|emb|CAJ01507.1| hypothetical protein [Manduca sexta]
07639 & 811 0 1616 18 2445 3912.5 186.0 gi|134436|sp|P14754.1| alaserpin or serpin-1 (07639, 15891)
07671 227 3 450 3 683 365.0 310.8 gi|195164814|ref|XP_002023241.1| GL21066 [Drosophila persimilis]
08076 & 47 3 115 2 167 75.6 119.1 gi|226342878|ref|NP_001139701.1| serpin 7 [Bombyx mori] (08076, 14528)
08224 etc. 7528 8 10093 0 17629 4539.6 20909.2 gi|1168527|sp|P14297.2| arylphorin β subunit (08224, 16474, 16501, 16664, 16715, 16764, 18695)
08467 0 0 113 0 113 0.0 234.1 gi|112983866|ref|NP_001036858.1| T7 lysozyme-like protein 1 (BTL-LP1) [Bombyx mori]
08500 138 0 407 0 545 665.7 843.2 gi|156406857|ref|XP_001641261.1| predicted protein [Nematostella vectensis]
08821 246 0 436 2 684 1186.8 451.6 gi|112983550|ref|NP_001036879.1| fibrillin-like protein [Bombyx mori]
08845 27 0 130 0 157 130.3 269.3 gi|195029763|ref|XP_001987741.1| GH19797 [Drosophila grimshawi]
08854 & 302 5 5234 0 5541 291.4 10843.3 gi|5869985|emb|CAB55603.1| moderately Met-rich storage protein [Spodoptera litura] (08854, 15324)
09928 30 0 106 0 136 144.7 219.6 gi|242090851|ref|XP_002441258.1| hypothetical SORBIDRAFT_09g023310 [Sorghum bicolor]
10071 & 15 1 1243 0 1259 72.4 2575.1 gi|228382|prf||1803340A Met-rich storage protein SP1A (10071, 17516)
10326 284 4 299 11 598 342.5 56.3 gi|56462160|gb|AAV91363.1| hypothetical protein 10 [Lonomia obliqua]
10791 etc. 2 0 2756 2 2760 9.6 2854.8 gi|4262357|gb|AAD14591.1| scolexin A [Manduca sexta] (10791, 10792, 16520, 18669, 18670, 18963)
11039 etc. 13962 11 19836 0 33809 6123.3 41094.2 gi|114240|sp|P14296.1| arylphorin α subunit (11039 16171 16537 16814 17492 18240 18257 18556)
11830 26 1 33 0 60 125.4 68.4 gi|260780799|ref|XP_002585527.1| hypothetical protein BRAFLDRAFT_89257 [B. floridae]
11922 & 901 12 1052 3 1968 362.2 726.5 gi|114058|sp|P13276.1| apoLp-III (11922, 13093)
12005 154 0 2177 0 2331 742.9 4510.1 gi|2625150|gb|AAB86646.1| moderately Met-rich hexamerin precursor [Hyalophora cecropia]
12151 0 0 153 0 153 0.0 317.0 gi|116084|sp|P14665.1|bactericidin B-5P, cecropin-like peptide precursor
12749 135 0 1462 0 1597 651.3 3028.8 gi|159530|gb|AAA29322.1| Met-rich storage protein 3 [Manduca sexta]
13563 0 0 657 0 657 0.0 1361.1 gi|110347786|gb|ABG72695.1| attacin-like protein [Antheraea mylitta]
13916 etc. 3 0 1327 0 1330 14.5 2749.1 gi|219958086|gb|ACL68097.1| lebocin-related protein precursor [M. sexta] (13916, 17301, 17434)
13994 57 0 62 0 119 275.0 128.4 gi|112983654|ref|NP_001036872.1| bombyrin [Bombyx mori]
14173 45 0 32 0 77 217.1 66.3 gi|153792114|ref|NP_001093267.1| phosphatidylethanolamine binding protein [Bombyx mori]
14375 etc. 400 0 681 0 1081 1929.7 1410.8 gi|400673|sp|P31420|OMBP ommochrome-binding protein precursor (14375, 14659, 17494, 17813)
14380 etc. 0 1 408 3 412 0.0 281.8 gi|67906420|gb|AAY82587.1| attacin-1 [Manduca sexta] (14380, 14641, 16150)
14700 & 0 0 301 4 305 0.0 155.9 gi|260765453|gb|ACX49764.1| peptidoglycan recognition protein 2 [Manduca sexta] (14700, 14752)
15089 271 0 194 0 465 1307.4 401.9 gi|158293921|ref|XP_315269.4| AGAP011516-PA [Anopheles gambiae]
15639 10 0 109 0 119 48.2 225.8 gi|148298818|ref|NP_001091784.1| multi-binding protein [Bombyx mori]
16000 61 0 138 0 199 294.3 285.9 gi|109458629|ref|XP_001073545.1| hypothetical protein [Rattus norvegicus]
16223 22 1 47 2 72 106.1 48.7 gi|242003442|ref|XP_002422733.1| bifunctional purine biosynthesis protein [Pediculus corporis]
16281 & 358 0 541 0 899 1727.1 1120.8 gi|134103857|gb|ABO60878.1| cationic peptide CP8 precursor [Manduca sexta] (16281, 17312)
16849 134 0 541 0 675 646.4 1120.8 gi|114051738|ref|NP_001040426.1| alcohol dehydrogenase [Bombyx mori]
17199 42 2 33 4 81 101.3 17.1 gi|3108073|gb|AAC15763.1| putative multifunctional protein ADE2 [Manduca sexta]
17350 0 0 205 0 205 0.0 424.7 gi|29469969|gb|AAO74640.1| antimicrobial protein attacin 2 [Manduca sexta]
18797 9 0 549 0 558 43.4 1137.4 gi|39843367|gb|AAR32136.1| VHDL receptor [Helicoverpa zea]
*

RA and ARN are calculated using original read numbers as described in Section 2.3. Listed here are contigs with RAIF/IH >100, RACF/CH >100, ARNIF >200 when RNIH =0, or ARNCF >200 when RNCH =0. RAIF/IH and RACF/CH values are shown in red if they are greater than 100, whereas ARNIF and ARNCF values are shown in blue if they are higher than 200. In the columns of RA or ARN, cells shaded green and orange represent down- and up-regulated gene expression, respectively. Contigs with identical BLAST results are combined, with their average RAs or ARNs calculated based on the sums of original reads in CF, CH, IF, and IH for each group. Contigs with no BLAST hit can be found in Table S4, a complete list of 250 FB CIFH contigs.