Figure 1.

Mck1 is required for calcineurin-dependent inhibition of Vcx1 function. Vcx1 function was measured by its ability to promote growth of Pmc1- and Tcn1-deficient cells in YPDS media supplemented with high concentrations of CaCl₂ as indicated. (A) The Vcx1-proficient pmc1 tcn1 double mutants (circles, strain K1349) and mck1 pmc1 tcn1 triple mutants (triangles, strain K1353) were inoculated at low density into media lacking (solid lines) or containing (dashed lines) FK506 and incubated for 18 h before measuring optical density at 650 nm (OD₆₅₀). (B) As a reference, the Vcx1-deficient pmc1 tcn1 vcx1 triple mutant (circles, strain K1357) and mck1 pmc1 tcn1 vcx1 quadruple mutant (triangles, strain K1361) were analyzed as in A.