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. Author manuscript; available in PMC: 2011 Jul 25.
Published in final edited form as: Anticancer Res. 2010 Sep;30(9):3599–3610.

Figure 4.

Figure 4

DH-PMF induces apoptosis. A: Cells were treated with 100 μM DH-PMF for different times, stained with Live/Dead reagent, and incubated at 37°C for 30 min. KBM-5 cells were analyzed under a fluorescence microscope. B: KBM-5 cells were treated with 100 μM DH-PMF for different lengths of time, incubated with anti-annexin V antibody conjugated with FITC, and analyzed with a flow cytometer for early apoptotic effects. C: KBM-5 cells were treated with different concentrations of DH-PMF for 48 h. Cells were fixed, stained with TUNEL assay reagent, and then analyzed with a flow cytometer for apoptotic effects.