DH-PMF induces apoptosis. A: Cells were treated with 100 μM DH-PMF for different times, stained with Live/Dead reagent, and incubated at 37°C for 30 min. KBM-5 cells were analyzed under a fluorescence microscope. B: KBM-5 cells were treated with 100 μM DH-PMF for different lengths of time, incubated with anti-annexin V antibody conjugated with FITC, and analyzed with a flow cytometer for early apoptotic effects. C: KBM-5 cells were treated with different concentrations of DH-PMF for 48 h. Cells were fixed, stained with TUNEL assay reagent, and then analyzed with a flow cytometer for apoptotic effects.