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. 2004 Jan 1;18(1):62–75. doi: 10.1101/gad.276504

Figure 9.

Figure 9.

Reinitiation efficiency decreases if the rate of elongation is decreased. (A) Capped TPI/+8norm and TPI/2+8ter mRNAs were cotranslated at 15 μg/mL each and (B) capped TPI/+8norm mRNA was translated at 15 μg/mL in the presence of cycloheximide at the designated concentrations. The apparent reinitiation efficiency, unadjusted for leaky scanning (i.e., R/F), is shown below each lane in A, and the percentage of leaky scanning (L/F) below each lane in B. (C) The gel loadings of the same samples as in A were adjusted so that the yield of full-length product (F) from the TPI/+8norm mRNA would be approximately the same in each lane. The reinitiation efficiency, adjusted for leaky scanning, is given below each lane.