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Efficiencies as functions of cycle number. Di = 105 copies, Ei = 2.1×1011 copies, elongation period is 20 s (A), 10s (B) and 5s (C). (D) Serial dilution study - normalized DNA product as a function of cycle number. Di = 102, 103, 104 and 105 (as indicated in the legend), Ei = 12.6×1011 copies, tE = 20 s.
