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. Author manuscript; available in PMC: 2011 Jul 25.
Published in final edited form as: Nat Immunol. 2010 Oct 3;11(11):997–1004. doi: 10.1038/ni.1932

Figure 6.

Figure 6

p204 is required for VACV 70mer DNA-stimulated transcription factor activation. a, siRNA-treated RAW264.7 cells grown on glass coverslips were mock-transfected or transfected with 2.5 μg/ml VACV 70mer or poly(I:C) for 6 h, fixed and stained for NF-κB p65 (red) and IRF3 (green). Nuclei were visualised by DAPI (blue). b, Cells treated as in (a) were qualitatively examined to assess staining of either p65 (left panel) or IRF3 (right panel) in the nucleus. Cells showing nuclear staining were counted and expressed as a percentage of total number of cells. At least 200 cells were counted per sample. Shown is a representative of four independent experiments.