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. 2011 Jun 28;105(2):246–254. doi: 10.1038/bjc.2011.197

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Copyright © 2011 Cancer Research UK

This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made.The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material.If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit https://creativecommons.org/licenses/by/4.0/.

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Mutation analysis of K-Ras codons 12, 13 and 146. Mutation detection was performed by dideoxy and pyrosequencing analyses, as described in the ‘Materials and methods’ section. Mutation status in samples analysed by dideoxy sequencing was assigned by automated base calling using 4Peaks software, and is shown in comparison with quantitative analysis of mutation burden, assessed by pyrosequencing. Representative analyses are illustrated.