Table 2. Summary of mutation frequencies in K-Ras, B-Raf and PIK3CA as analysed by dideoxy and pyrosequencing.

			Frequency (%)
Mutation	Nucleotide change	Amino-acid change	Dideoxy sequencing	Pyrosequencing
K-Ras			26.4%	32.4%
Codon 12	G34A	Gly12Ser	1/102 (1%)	3/102 (2.9%)
	G34T	Gly12Cys	1/102 (1%)	4/102 (3.9%)
	G35T	Gly12Val	8/102 (7.8%)	9/102 (8.8%)
	G35C	Gly12Arg	1/102 (1%)	1/102 (1%)
	G35A	Gly12Asp	8/102 (7.8%)	8/102 (7.8%)
Codon 13	G38A	Gly13Asp	6/102 (5.9%)	6/102 (5.9%)
Codon 61	None detected	None detected	0/102	0/102
Codon 146	G436A	Ala146Thr	2/102 (1.9%)	2/102 (1.9%)
Codon 14	Ins 41–44	14Gly15	1/102 (1%)	1/102 (1%)
Codon 22	C65A	Gln22Lys	1/102 (0.6%)	1/102 (0.6%)
B-Raf			8.8%	11.5%
Codon 600	T1798A	Val600Glu	9/102 (8.8%)	12/102 (11.5%)
PIK3CA			8.8%	13.70%
Codon 542	G1624A	Glu542Lys	4/102 (3.9%)	4/102 (3.9%)
Codon 545	G1633A	Glu545Lys	3/102 (2.9%)	7/102 (6.8%)
Codon 546	A1637C	Gln546Pro	1/102 (1%)	1/102 (1%)
Codon 1023	None detected	None detected	0/102	0/102
Codon 1047	A3140G	His1047Arg	1/102 (1%)	2/102 (1.9%)