Figure 2.

GO-Y030 inhibited STAT3 phosphorylation and downregulated STAT3-regulated genes expression in ALDH⁺/CD133⁺ cells. (A) ALDH⁺/CD133⁺ cells were treated with DMSO or 5 μM of GO-Y030 for 24 h. Phosphorylation of STAT3 in DLD-1, HCT-116, and SW480 colon cancer stem cells were detected by western blot. (B) ALDH⁺/CD133⁺ cells were treated with DMSO or 50 μM of curcumin for 24 h. (C) ALDH⁺/CD133⁺ cells were treated with GO-Y030 (5 μM) or DMSO for 24 h. Reverse transcriptase–polymerase chain reaction reveals decreased expression of STAT3 downstream target genes in GO-Y030-treated cells as compared with DMSO control.