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. 2011 Jun 21;105(2):212–220. doi: 10.1038/bjc.2011.200

Table 1. ALDH+/CD133+ cells generated more tumourspheres than ALDH/CD133 cells.

  SW480 HCT-116 DLD-1 HT29
250 cells per well        
 ALDH+/CD133+ 2±1* 2±0* 19±3* 12±2*
 ALDH/CD133 0 0 0 1±1
         
500 cells per well        
 ALDH+/CD133+ 7+1* 8±2* 29±10* 21±3*
 ALDH/CD133 0 2±0 1±1 3±2
         
1000 cells per well        
 ALDH+/CD133+ 8±2* 12±2* 42±9 * 21±2*
 ALDH/CD133 3±1 2±1 1±1 5±1

ALDH+/CD133+ and ALDH/CD133 subpopulations of colorectal cancer cells were separated by flow cytometry and cultured in stem cell medium as described in Materials and Methods. The numbers of tumoursphere generated per 250, 500, or 1000 cells were counted 2 weeks later. *P<0.01.