Table 1. ALDH+/CD133+ cells generated more tumourspheres than ALDH−/CD133− cells.
SW480 | HCT-116 | DLD-1 | HT29 | |
---|---|---|---|---|
250 cells per well | ||||
ALDH+/CD133+ | 2±1* | 2±0* | 19±3* | 12±2* |
ALDH−/CD133− | 0 | 0 | 0 | 1±1 |
500 cells per well | ||||
ALDH+/CD133+ | 7+1* | 8±2* | 29±10* | 21±3* |
ALDH−/CD133− | 0 | 2±0 | 1±1 | 3±2 |
1000 cells per well | ||||
ALDH+/CD133+ | 8±2* | 12±2* | 42±9 * | 21±2* |
ALDH−/CD133− | 3±1 | 2±1 | 1±1 | 5±1 |
ALDH+/CD133+ and ALDH−/CD133− subpopulations of colorectal cancer cells were separated by flow cytometry and cultured in stem cell medium as described in Materials and Methods. The numbers of tumoursphere generated per 250, 500, or 1000 cells were counted 2 weeks later. *P<0.01.