Table 1. Primers and siRNA oligomers used.

Gene	Primer sequences a
Hoxc8 human	5′-CAT GTT TCC ATG GAT GAG ACC-3′ forward 5′-TGA TAC CGG CTG TAA GTT TGC-3′ reverse
OSTEONECTIN (ON, SPARC) human	5′-CCC GCT TTT TCG AGA CCT-3′ forward 5′-CAA GAT CCT TGT CGA TAT CCT TC-3′ reverse
OSTEOPONTIN (OPN, SPP1) human	5′-CGC AGA CCT GAC ATC CAG T-3′ forward 5′-GGC TGT CCC AAT CAG AAG G-3′ reverse
GAPDH human	5′-AGC CAC ATC GCT CAG ACA-3′ forward 5′-GCC CAA TAC GAC CAA ATC C-3′ reverse
siRNA	siRNA sequencesb
siRNA Hoxc8 negative control 1	5′-GAGUCCGAACCCUUAGUAUGCACUA-3′ 5′-UAGUGCAUACUAAGGGUUCGGACUC-3′
siRNA Hoxc8-1	5′-GAGACGCCUCCAAAUUCUAUGGCUA-3′ 5′-UAGCCAUAGAAUUUGGAGGCGUCUC-3′
siRNA Hoxc8 negative control 2	5′-UGGUCAGCCAGAGAGACGAUAGGAA-3′ 5′-UUCCUAUCGUCUCUCUGGCUGACCA-3′
siRNA Hoxc8-2	5′-UGGGACUGACCGAGAGACAAGUGAA-3′ 5′-UUCACUUGUCUCUCGGUCAGUCCCA-3′

Abbreviation: SPARC=secreted protein acidic and rich in cysteine.

^aObtained from Dharmacon (Lafayette, CO, USA).

^bObtained from Invitrogen.