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. Author manuscript; available in PMC: 2011 Jul 25.
Published in final edited form as: J Immunol. 2009 Oct 15;183(8):4895–4903. doi: 10.4049/jimmunol.0901459

FIGURE 4. Correlation between Ag dose, pS6 and Foxp3 expression.

FIGURE 4

A–C, CD4+ BDC2.5 T cells were stimulated with G4DC or GMDC, plus high-affinity (AV10) or low-affinity (KV11) peptides, at the indicated doses. T cells were stained at 18hrs for CD4 and intracellular pS6, and at d7 for CD4 and intracellular Foxp3. A, % Foxp3+ cells in CD4+ gate at d7. B and C, pS6 MFI at 18hrs (x-axis) and % Foxp3+ at d7 (y-axis). D and E, CD4+ BDC2.5 T cells were stimulated for with plate-bound anti-CD3 at indicated doses, plus soluble anti-CD28 at 1ug/ml. T cells were stained at 18hrs for CD4 and intracellular Phospho-S6, and at d5 for CD4 and intracellular Foxp3. D, Proliferation and Foxp3 expression in T cells after 5 days of stimulation. E, P-S6 levels at 18hr and % Foxp3 at day5. Data shown in all panels are representative of ≥3 independent experiments.