Skip to main content
. Author manuscript; available in PMC: 2011 Jul 25.
Published in final edited form as: Cell Host Microbe. 2009 Mar 19;5(3):234–243. doi: 10.1016/j.chom.2009.02.005

Figure 1. The ACD of VgrG-1 is required for host cell phenotypes.

Figure 1

A. Western blots of pellet and supernatant fractions of various V52 strains. B. Western blot of host cell actin from J774 cells incubated with various V52 strains at an MOI of 10 for 2 hours. C. Supernatants of J774 cells were analyzed for LDH release one day after exposure to V. cholerae. Values are from triplicate wells and are expressed as percentage of LDH released from lysed mock-treated cells. Error bars indicate +/− one standard deviation. D. Fluorescence microscopy of cells visualizing actin with rhodamine-phalloidin (red) and nuclei with DAPI (blue). Scale bar indicates 10 μm. E. Dictyostelium discoideum plaque assay with various V52 strains. F. Quantification of plaque formation normalized to plaque formation on K. aerogenes.