Table 3.
Performance Features of Initial and Optimized Set Represented by a Combined Score for Cy3 and Cy5 Channels
|
Initial set
|
Removed clones
|
Optimized set
|
|||||
|---|---|---|---|---|---|---|---|
| Quality parameter | Number filtereda | Mean | Std. dev. | Mean | Std. dev. | Mean | Std. dev. |
| Signal variance | 148 | 0.5 | 0.1 | 0.3 | 0.1 | 0.5 | 0.1 |
| Mean-median correlation | 73 | 0.8 | 0.2 | 0.3 | 0.8 | 0.8 | 0.1 |
| Percent pixels >2 SD above background | 203 | 88.8 | 10.2 | 58.2 | 13.8 | 90.1 | 7.1 |
| Ratio std. dev. >0.2 | 336 | 1.1 | 0.5 | 1.5 | 1.4 | 1 | 0.1 |
| Total | 373 | ||||||
a
Many clones were removed based on multiple criteria