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. Author manuscript; available in PMC: 2012 Jul 1.
Published in final edited form as: Biochim Biophys Acta. 2011 Apr 22;1811(7-8):452–459. doi: 10.1016/j.bbalip.2011.04.005

Figure 5. Selective proteolysis of radiolabeled wild type IFABP.

Figure 5

After hydrophobic photolabeling with 125I-TID-PC, apo-IFABP was blotted and subjected to selective proteolisys with BNPS-Skatole. The results showed a preferential radiolabeling (right panel) of the fragment containing the α-helical region (9.6 kDa) than the one corresponding to the second half of the β-barrel (5.5 kDa) compared to the coomasie blue staining (left panel).