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. 2000 Jul 15;106(2):217–224. doi: 10.1172/JCI7132

Figure 2.

Figure 2

Inhibition of mAb 3.B6 by HCM and GlcNAc-BSA. Monoclonal antibody 3.B6 (10 μg/mL) was mixed 1:1 with increasing amounts of inhibitor (0 to 500 μg/mL), and binding of free mAb to immobilized HCM (a) or GlcNAc-BSA (b) on the microtiter plate was detected by ELISA. The concentration of HCM required to inhibit 50% of binding of mAb 3.B6 to GlcNAc-BSA was 67 μg/mL (b). The concentration of GlcNAc-BSA required to inhibit 50% of binding of mAb 3.B6 to HCM was more than 500 μg/mL (a). BSA alone did not inhibit mAb 3.B6. Inhibitors are as follows: squares, HCM; diamonds, GlcNAc-BSA; circles, BSA. Percentage of inhibition was calculated using the following formula: 100 × (1-[A410 inhibitor + mAb 3.B6/A410 PBS + mAb 3.B6]).