Figure 3.

Loss of subtype specification in Brn3a/Islet1 DKO embryos. A, Markers of sensory subtypes were examined by immunofluorescence in matched sagittal sections of cervical DRGs from Brn3a KO, Islet1 CKO, DKO, and control E13.5 embryos. B, Key markers of sensory subtype differentiation were examined in the TG of control and DKO ganglia at E13.5. Horizontal sections are shown. TrkB expression is restricted to specific cells in control ganglia; TrkB expression persists in DKO ganglia but no longer identifies a specific set of cell bodies. Dashed lines indicate location of the DRG in sections in which the fluorescence signal cannot be distinguished from background.