Table 2.
Primers used for amplification and sequencing of the four chloroplast intergenic spacer regions
| Region/primer | Sequence (5'-3') | Use | Reference |
|---|---|---|---|
| psbD-trnT | |||
| psbD-F | TCAACTACTTCAACCATTTCC | Amplification/sequencing | Ye Tun Tun and Yamaguchi (2008) |
| trnT-R | TGGTAAGGCGTAAGTCATCG | Amplification/sequencing | Ye Tun Tun and Yamaguchi (2008) |
| DT-F2 | TGGTGGAACTTGAAATTGGT | Sequencing | Ye Tun Tun and Yamaguchi (2008) |
| DT-R2 | ACCAATTTCAAGTTCCACCA | Sequencing | Ye Tun Tun and Yamaguchi (2008) |
| trnT-trnE | |||
| trnT-F | CGATGACTTACGCCTTACC | Amplification/sequencing | Ye Tun Tun and Yamaguchi (2008) |
| trnE-R | AGAGAGATGTCCTGAACCAC | Amplification/sequencing | Ye Tun Tun and Yamaguchi (2008) |
| petA-psbJ | |||
| petA-F | GTTACGTGTCCAAGGTCTC | Amplification/sequencing | This study |
| psbJ-R | CCGATACTACTGGAAGGATT | Amplification/sequencing | This study |
| trnT-trnL | |||
| trn-a | CATTACAAATGCGATGCTCT | Amplification/sequencing | Taberlet et al. (1991) |
| trn-b | TCTACCGATTTCGCCATATC | Amplification/sequencing | Taberlet et al. (1991) |