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. 2011 Jun 20;2:9. doi: 10.1186/1759-8753-2-9

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Copyright ©2011 Kahlon et al; licensee BioMed Central Ltd.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Herves-L bp 12-48 and bp 28-60 are important for transposase binding. Electrophoretic mobility shift assays (EMSAs) with (a) Herves- left (L) bp 1-100 and (b) Herves-L bp 12-48 as probes. Overlapping 30 bp fragments were used as competitors for transposase binding to the probe. The specific and non-specific competitors were use at 200-fold molar excess to the probe, unless specified otherwise. The asterisk (*) indicates various protein DNA complexes. (a) Specific and non-specific competitors was used as described for Figure 1; (b) a 30 bp non-homologous fragment, from the β2 tubulin gene of Aedes aegypti, was used as a non-specific competitor.