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. 2011 Jun 20;2:9. doi: 10.1186/1759-8753-2-9

Figure 3.

Figure 3

Transposase binding analysis to the Herves-left (L) end. The single-end-labeled Herves-L 1-100 bp fragment (100 nM) was incubated in presence (+, ++) or absence (-) of DNase I or the transposase. The ++ indicates 1.4 μM of transposase or 0.2 units of DNase I, whereas + indicates 850 nM of transposase or 0.1 units of DNase I. 32P indicates the position where probe was labeled. The solid bars indicate regions protected by the transposase from DNase I degradation. The red asterisk (*) indicates hypersensitive sites.