Figure 6.
The CGATTCAT binding motif is sufficient for transposase binding. The probe (CGATTCAT)4 represents four direct repeats of the CGATTCAT sequence motif. Unlabeled (CGATTCAT)4 and Herves-left (L) bp 28-60 were used as specific competitors, whereas β2 tubulin was used as the non-specific competitor. The transposase binding was compared between the sequence variants of the binding motif such as CGATTCTT, CGATTCAC and CGTTCAT (each used as four direct repeats). The asterisk (*) indicates various protein DNA complexes.