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. 2011 Jul 25;6(7):e21996. doi: 10.1371/journal.pone.0021996

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Narayanan et al.

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

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(A) Measurement of total protein hydrolyzed and free amino acid concentrations in transgenic and wild-type roots. Samples were hydrolyzed with HCl and subjected to ACQUITY UPLC® System. Data are presented as nmoles/mg total dry weight. Error bars indicate SE of the mean of two biological replicates. The asterisk (*) indicates statistically significant differences between wild-type and transgenics, determined by Student's t-test, with P<0.05. (B) Measurement of free amino acid concentrations of transgenic and wild-type roots. Data are presented as nmoles/mg total dry weight of tissue. Error bars indicate SE of the mean of two biological replicates.