Figure 6. Using the Coupling and Electron Flow assays in tandem to elucidate mechanistic activity of test agents.

Coupling (A, C) and electron flow experiments (B, D) were performed as described in Methods. Initial conditions are as follows (with final concentrations listed): A–B, Controls (no additives) or 20 mM sodium azide or 4 µM antimycin-A; C–D, Controls (no additives) or 10 mM malonate or 2.5 µg/ml oligomycin or 2 µM rotenone. See text for further explanation of results.