Table 3. Survival after a stress of one hour at pH 2.3 when ΔadiAΔcadAΔspeF is complemented by cadA.
Strain | Available substrate | |||
None | Arginine | Lysine | Ornithine | |
WT pACYC177 | 0.6±0.4 | 74±25 | 33±9 | 10±0.7 |
WT p cadA | 2±2 | 86±42 | 32±11 | 24±6 |
Δ adiA Δ cadA Δ speF pACYC177 | 0.07±0.1 | <0.05 | <0.05 | 0.08±0.08 |
Δ adiA Δ cadA Δ speF p cadA | 2±1.7 | 1.8±0.2 | 35±11 | 2±1.8 |
Values in the table are percentage survival ± standard deviation, which results from three independent experiments. Viability was assessed by CFU count on LB plates. The percentage survival represents the ratio between the CFU counted after and before stress. Cultures and stresses were done in anoxic conditions. When indicated 20 mM L-Arg, L-Lys or L-Orn were added to the medium during the challenge.