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. 2011 Jul 25;6(7):e22465. doi: 10.1371/journal.pone.0022465

Figure 11. Distribution of metalloproteinases in HUCB-NSCs.

Figure 11

Equivalent numbers of HUCB-NSCs were plated on fibronectin-coated coverslips and grown 8 days in culture in serum-free medium. Confocal photomicrographs show co-staining of in situ gelatinolytic activity (green) with neuronal markers (red): Tuj1 and MAP2, the astrocytic marker S100beta and oligodendrocytes marker GalC. Double staining appears in yellow. The cell nuclei counterstained with Hoechst (blue). Note the metalloproteinase activity in all tested phenotypes. Photomicrographs are representative of observations made from at least three independent experiments. Scale bar 20 µm.