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. 2011 Jul 25;6(7):e22409. doi: 10.1371/journal.pone.0022409

Figure 2. Spectroscopic interaction evidence of αE2-conj and DNA.

Figure 2

A. Near-UV CD spectrum at 25°C of the oligonucleotide hemisite E2-Dst (2 µM) alone, after the addition of 10 µM Dst or αE2- conj. B. Far-UV CD spectrum of αE2- conj alone, in the presence of hemisite E2-Dst, hemisite E2-non Dst, hemisite non E2-Dst or hemisite non E2-non Dst. C. Far-UV CD spectrum of αE2- conj alone and in the presence of hemisite E2-Dst (upper panel), and of αE2- Ala alone and in the presence of hemisite E2-Dst (lower panel). CD measurements in B and C were performed at 5°C in solutions containing 10 mM phosphate buffer pH 7.0, 100 mM NaCl, and 5 µM peptide after incubating 20 minutes at room temperature with 5 µM ds-oligonucleotide, when present. B and C show the spectrum of the mixture (peptide and DNA) minus the spectra of the corresponding DNA alone.