. 2011 Jul 25;6(7):e22464. doi: 10.1371/journal.pone.0022464

− mice^a.

		Lymphocyte number (×10⁻⁶)
Tissue	Phenotype	WT	CD22^−/−	CD154^TG	CD154^TGCD22^−/−
BM:	B220^hiIgM^hi	1.5±0.3	0.4±0.2*	1.2±0.3	0.5±0.03*
	B220^hiHSA^lo	1.4±0.2	0.3±0.1**	1.4±0.3	0.3±0.04**
Blood:	B220⁺	2.7±1	0.9±0.2*	3.9±1	0.4±0.1**
Spleen:	B220⁺IgM⁺	68±9	46±8	250±40**	171±20**
	B10^b	1.7±0.4	1.8±0.4	6.5±2**	26.7±6**
	B10+B10pro	6.1±2	5.4±0.8	24±4**	66±3**
	IgM⁺IgD⁺CD23^hi	56±8	42±7	224±50*	127±20*
	IgM^hiCD21^hi	5.6±0.9	1.4±0.3**	24±4**	29±6*
	CD1d^hiCD5⁺	1.8±0.4	2.3±0.8	4.2±0.3**	31±5*
	B220^loCD5⁺	2.2±0.5	1.7±0.4	9.2±3*	6.6±1*
	Thy1.2⁺	39±5	38±7	39±4	53±9
	CD4⁺Foxp3⁺CD25⁺	3.4±0.2	3.3±0.4	2.7±0.4	4.4±0.8
	CD4⁺ T naïve	15±4	18±5	11±2	8.5±4
	CD4⁺ T memory	6.9±0.8	5.8±0.8	9.7±0.6*	14±3*
	CD8⁺ T naïve	13±2	14±3	5.9±1*	2.0±0.6**
	CD8⁺ T memory	0.7±0.08	0.8±0.1	9.1±1**	15±3**
	IL-10⁺ T cells^c	0.6±0.2	0.8±0.2	6.4±2.5*	8.1±2.2**
PLN:	B220⁺	4.6±0.8	1.9±0.2*	9.4±1*	4.4±2
	B10	0.01±0.005	0.003±0.001	0.06±0.01*	0.2±0.1*
MLN:	B10	0.02±0.01	0.02±0.003	0.3±0.06**	0.4±0.2*
PC:	B220⁺IgM⁺	0.7±0.1	0.3±0.1	2.9±0.6*	1.2±0.3
	B10	0.6±0.1	0.5±0.1	0.7±0.1	0.7±0.3
	B220^loCD5⁺	0.4±0.1	0.3±0.1	1.0±0.3	0.4±0.1
	B220^hiIgD^hi	0.5±0.1	0.2±0.1	2.4±0.5*	1.0±0.2

Numbers represent mean (±SEM) values from ≥4 littermates of each genotype. Asterisks indicate values significantly different from WT mice (*p<0.05; **p<0.01). PLN, peripheral LN; MLN, mesenteric LN; PC, peritoneal cavity.

B10 and B10pro cells were identified as in Figure 3.

IL-10-competent T cells were identified following 5 h stimulation with PMA/ionomycin.

Table 1. Lymphocyte subsets in WT, CD22−/−, CD154TG, and CD154TGCD22−/− micea.

Table 1. Lymphocyte subsets in WT, CD22^−/−, CD154^TG, and CD154^TGCD22^−/− mice^a.