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. 2011 Jul 25;6(7):e22559. doi: 10.1371/journal.pone.0022559

Figure 1. Stable knockdown of GRK2, GRK3, GRK5 and GRK6 in human mast cells.

Figure 1

HMC-1 cells were stably transduced with scrambled shRNA control lentivirus (Control) or shRNA lentivirus targeted against GRK2, GRK3, GRK5 and GRK6. (A–D) Quantitative PCR was performed to assess GRK2, GRK3, GRK5 and GRK6 mRNA levels in shRNA control and GRK knockdown (KD) cells. Results are expressed as a ratio of GRK to GAPDH mRNA levels. Data represent the mean ± SEM from three independent experiments. Statistical significance was determined by unpaired two-tailed t test. ***, p<0.001. Representative immunoblots of HMC-1 cells with knockdown of (E) GRK2 and (F) GRK5/GRK6 are shown.