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. 2011 Jul 25;6(7):e22553. doi: 10.1371/journal.pone.0022553

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Li et al.

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

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Supernatants for TCID₅₀ tests from Vero E6 cells (A) and C6/36 cells (B), which had been exposed to DENV1 to 4 for 2, 4, 6, 8, 10, 15, 20 days, were collected and analyzed for NS1 production by ELISA. Data are averaged from 3 separate experiments. Results are shown as means ± standard deviations.