Table 3.

Relative mRNA abundance of CYP1C1 in developing zebrafish exposed to TCDD and BNF.

Hours post fertilization	Control	TCDD	BNF
6	24 ± 8.1	120 ± 40*	190 ± 74*
12	90 ± 43	200 ± 62	230 ± 120*
24	150 ± 52	270 ± 94	490 ± 280*
36	160 ± 56	3000 ± 1200*	4700 ± 1300*
48	320 ± 170	8300 ± 2200*	3400 ± 760*
72	430 ± 130	13000 ± 14000*	1100 ± 610
96	430 ± 43	5700 ± 3500*	1200 ± 340

Embryos were exposed to vehicle (DMSO), 0.5 ppb TCDD or 0.2 μM BNF, and euthanized at each developmental stage for total RNA isolation. cDNAs converted were used for quantitative real-time PCR. Elongation factor 1α (EF1α) was used as a reference gene. Units are CYP1C1 transcript/EF1α × 1,000. Results are expressed as mean ± SD. N= 5 per group. Significance of differences in the mean mRNA expression levels between control and treatment groups (i.e., TCDD or BNF) in each developmental stage was determined by one-way ANOVA followed by Dunnett test. Asterisks indicate significant (p < 0.05) difference as compared to respective controls.