Figure 2.

TNF-α decreases T₃-dependent increase of 5′-DI enzyme activity. (a) HepG2 cells were treated with T₃ (100 nM) and/or TNF-α (100 U/mL) for 24 hours. The enzyme activity of 5′-DI in cytosolic extract was expressed as mean ± SD (n = 6). (b) The fold induction of 5′-DI enzyme activity by T₃ is depicted. ^AP < 0.0001 vs. T₃ (–); ^BP < 0.0001, T₃ (+), TNF-α (–) vs. T₃ (+), TNF-α (+) in a; ^CP < 0.0001 vs. TNF-α (–) in b.