Figure 9.
Insulin treatment reverses delayed gastric emptying. (a) Insulin treatment (1 week) of STZ-diabetic and NOD-diabetic mice reverses delayed gastric emptying (20% dextrose). The data shown are the means (± SEM) of quadruplicate determinations representing four animals for each data point. (b) Inhibition of nNOS with 7-NI delays gastric emptying in insulin-treated diabetic mice. Diabetic mice were treated with insulin (1 week) and subsequently treated with the nNOS inhibitor 7-NI (50 mg/kg) as described in Methods. Gastric emptying was measured, and mice were sacrificed at 30 minutes. Data shown are means (± SEM) of five determinations reflecting five animals in each group. AP < 0.01 for 7-NI–injected, insulin-treated NOD-diabetic animals compared with insulin-treated NOD-diabetic mice and for 7-NI–injected, insulin-treated STZ-diabetic animals compared with insulin-treated STZ-diabetic animals.