Amplification of the intergenic region of the mini-exon genes of Trypanosoma cruzi. with primers TCC and TC 1; lanes (1–3) negative controls: (1) M5631, reference strain of TC IIc; (2) CanIII, reference strain of TC IIa; (3) X-10, reference strain of TC I; lanes (4–5) negative controls; lane (6) domestic dog TC IIc, with no amplification; lanes (7–10) TC IIc, yielding the 250 bp band pattern ; (7) peridomestic Tr. infestans; (8–9) domestic dogs; (10) Conepatus chinga, skunk; (11) CL Brener, reference strain of TC IIe; (12) 100 bp ladder.