Classical activation is suppressed in p53−/− microglia. A) Q-PCR results from p53+/+ and p53−/− microglia treated with IFNγ or control for interleukin-1α (IL-1α), interleukin-1β (IL-1β) and the murine scavenger receptor MARCO. All three classical activation mRNA’s were significantly lower following IFNγ stimulation in p53−/− microglia (*=p<0.05, **=p<0.01 by two-way ANOVA post-hoc comparison, n=4). B) Secretion of the pro-inflammatory cytokines TNFα and interleukin-12 (IL-12) was enhanced by IFNγ treatment in p53+/+ microglia, but p53−/− microglia failed to respond to IFNγ stimulation by increasing release of these pro-inflammatory cytokines. (*=p<0.05, **=p<0.01 by two-way ANOVA post-hoc comparison, n=3)