Table 3.

Oligonucleotide primers used to construct Pfu RPP29 derivatives

PfuP29Δ5-F	5′-cctagtccATGGAAGAACGTGAGAATAGAACTTCAG-3′
PfuP29Δ13-F	5′-gctattccATGGGGAGATCACAGGGATCG-3′
PfuP29Δ17-F	5′-cctcgaccATGGGATCGTATCAAGAAATTATTGG-3′
PfuP29Δ24-F	5′-gcatttccATGGGTAGAACTTGGATTTTCAGAGG-3′
PfuP29Δ31-F	5′-cctaaaccATGGGAGCTCATAGAGGTAGAGTAAAC-3′
PfuP29Δ36-F	5′-cgtggaccATGGGTAGAGTAAACAAGAAAAATATAGTATGG-3′
PfuP29Δ42-F	5′-cgaattccATGGGCAATATAGTATGGCACGAACTAATC-3′
PfuP29C127-R	5′-cctaactcgagTCATTTACGCCAACGC-3′

Start and stop codons are highlighted in bold, and NcoI (in Forward primers, denoted as F) and XhoI (in Reverse primer, denoted as R) restriction sites are underlined. Uppercase letters correspond to the open reading frame.