Fig. 3.

Palladin localizes at actin attachment sites at cell membrane dense bodies in SM tissue and their equivalent, focal adhesions in cultured rat aortic SMCs (Jin et al. 2007). a Both palladin and LPP localize in focal adhesions of cultured SMCs (R518, rat aortic smooth muscle cells). Unlike LPP, palladin also localizes periodically along the stress fibers with α-actinin (not shown) in cultured SMCs. b Note the periodic localization of both palladin and its partner protein, LPP around the periphery of bladder SMCs at the plasma membrane dense bodies. Cultured rat aortic SMC and Guinea pig bladder were fixed and immunostained with antibodies against palladin (monoclonal) and LPP (polyclonal). The signals were detected with corresponding secondary IgG conjugated with Alexa fluor 488 or 594 and visualized with a confocal microscope