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. Author manuscript; available in PMC: 2012 Aug 1.
Published in final edited form as: Alcohol Clin Exp Res. 2011 Apr 4;35(8):1435–1444. doi: 10.1111/j.1530-0277.2011.01479.x

Fig. 6.

Fig. 6

Ethanol decreases lipid raft colocalization of Lck and LAT in Jurkat cells. Jurkat cells were untreated (U), treated for 24 hours with 25 mM ethanol alone (E), 5 μg/ml phytohemagglutinin (PHA) alone for 5 minutes (PHA), or ethanol followed by PHA (E+PHA). Detergent-resistant lipid raft domains were isolated from Triton-X100 solubilized Jurkat cells using sucrose density gradient ultracentrifugation. Lipid raft colocalization of Lck and LAT, as well as raft-resident marker Flotillin, was analyzed by immunoblotting of raft (#2 to #4) and nonraft (#7 to #8) fractions using specific antibodies.