Table 1.
Velocity of anterograde transport in CEM cilia (µm/s)
| Ciliary kinesins |
||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Kinesin-3 |
Kinesin-2 |
|||||||||||
| GFP∷KLP-6 | KAP-1∷GFP | OSM-3∷GFP | ||||||||||
| Genotype | Mean ± sd | n | N | PpN | Mean ± sd | n | N | PpN | Mean ± sd | n | K | PpN |
| Wild type | 0.72 ± 0.18a | 103 | 16 | 6.4 | 0.52 ± 0.12a | 137 | 34 | 4.0 | 0.73 ± 0.23a | 152 | 18 | 8.4 |
| osm-3 | 0.77 ± 0.14a | 82 | 16 | 5.1 | 0.48 ± 0.14a | 63 | 23 | 2.7 | NA | |||
| klp-6; osm-3 | NA | 0.49 ± 0.09a | 87 | 25 | 3.5 | NA | ||||||
| klp-6 | NA | 0.73 ± 0.14b | 116 | 29 | 4.0 | 0.89 ± 0.22b | 134 | 12 | 11.2 | |||
| klp-11 | 0.75 ± 0.17a | 66 | 13 | 5.1 | NA | 0.92 ± 0.25b | 347 | 30 | 11.6 | |||
| klp-6; klp-11 | NA | NA | 1.07 ± 0.29c | 335 | 26 | 12.9 | ||||||
| IFT polypeptides |
||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|
| IFT-A particle |
IFT-B particle |
|||||||||||
| CHE-11∷GFP | OSM-5∷GFP | OSM-6∷GFP | ||||||||||
| Genotype | Mean ± sd | n | N | PpN | Mean ± sd | n | N | PpN | Mean ± sd | n | N | PpN |
| Wild type | 0.59 ± 0.14a | 197 | 38 | 5.2 | 0.57 ± 0.11a | 167 | 26 | 6.4 | 0.52 ± 0.13a | 184 | 44 | 4.2 |
| osm-3 | 0.45 ± 0.10b | 69 | 23 | 3.0 | 0.48 ± 0.15ab | 45 | 13 | 3.5 | 0.46 ± 0.15a | 81 | 23 | 3.5 |
| klp-6; osm-3 | 0.48 ± 0.10b | 136 | 25 | 5.4 | 0.44 ± 0.10b | 108 | 19 | 5.7 | 0.46 ± 0.11a | 104 | 20 | 5.2 |
| klp-6 | 0.67 ± 0.16c | 181 | 25 | 7.2 | 0.73 ± 0.19c | 173 | 22 | 7.9 | 0.68 ± 0.20b | 198 | 32 | 6.2 |
| klp-11 | 1.06 ± 0.25d | 185 | 29 | 6.4 | 0.92 ± 0.23d | 187 | 22 | 8.5 | 0.91 ± 0.27c | 220 | 32 | 6.9 |
| klp-6; klp-11 | 1.17 ± 0.19e | 233 | 25 | 9.3 | 1.07 ± 0.19e | 150 | 12 | 12.5 | 1.07 ± 0.23d | 286 | 38 | 7.5 |
Indicated are mean anterograde velocities ± SD of GFP-tagged ciliary kinesins and IFT particle proteins. Values within a column followed by a common letter are not significantly different at p<0.001 based on one-way Anova with Bonferroni post-hoc test. n=number of particles traced, N=number of cilia imaged, PpN = particles per cilium, NA = not available. Anterograde velocity could not be measured in klp-11 osm-3 double mutants due to severe ciliary abnormalities and large accumulations of reporters.