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. Author manuscript; available in PMC: 2012 Oct 1.
Published in final edited form as: Anal Biochem. 2011 May 30;417(1):142–148. doi: 10.1016/j.ab.2011.05.038

Figure 2.

Figure 2

Effects of washing and reaction temperature. A. In vitro oxidized or non-oxidized radiolabeled RNA (1.8 kb) with or without derivatization, was incubated with strep-beads at 37°C for 15min. The beads were washed with several buffer solutions including 4M urea, and then heat denatured to dissociate the bound RNA. Radioactivity in the pull-down fraction as well as initial input RNA was quantified using a PhosphorImager. B. In vitro oxidized radiolabeled ARP-RNA was incubated with strep-beads at different temperatures for 30 min. The beads were washed several times with salt buffers including 4M urea buffer solution and heat denatured. Radioactivity in the pull-down fraction and initial input RNA was determined. The results are represented as means ± SD from triplicate samples.