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. Author manuscript; available in PMC: 2012 Oct 1.
Published in final edited form as: Anal Biochem. 2011 May 30;417(1):142–148. doi: 10.1016/j.ab.2011.05.038

Figure 5.

Figure 5

Quantitative RT-PCR for total and pull-down RNA fraction. Total cellular RNA was isolated from Neuro-2a cells treated with 0.1mM or 1mM H2O2 for 30min. ARP-RNA (1μg of total ARP-derivatized RNA) was reverse transcribed with random hexamers (left panels) or oligo dT primers (right panels). The cDNA was subjected to real time PCR using three different sets of primers specific for β-actin (A) or eEF2 (B). Based on the Ct value, quantities of the gene transcripts were determined. X-axis is the 5’position of the forward primer. The graphs are represented as relative quantity compared to the 1035 primer (A) or 2441 primer (B), respectively and expressed as means±SD from tests in triplicate.