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. Author manuscript; available in PMC: 2012 Aug 15.
Published in final edited form as: Dev Biol. 2011 May 12;356(2):308–322. doi: 10.1016/j.ydbio.2011.05.583

Figure 7. Evaluation of OFT phenotypes following Cre/loxP-mediated genetic NC ablation in Wnt1-Cre;R26-EGFP-DTA embryos.

Figure 7

(A–C) Gross examination of E15 Wnt1-Cre;R26-EGFP-DTA (A), R26-EGFP-DTA only (B) and wildtype non-transgenic sibling controls (C) viewed under UV. Note lack of craniofacial structures (open arrowhead) and internalized eyes following genetic NC ablation (A). Insets show same embryos viewed under brightfield. The R26-EGFP-DTA non-transgenic sibling embryos are normal, indicative of absent non-specific DTA expression in non-appearance of the Wnt1-Cre transgene. (D–G) Subsequent transverse sectioning and counterstaining with either (D,E) αSMA immunohistochemistry (brown DAB staining) or (F,G) Hematoxylyn/Eosin (blue/pink) revealed that the Wnt1-Cre;R26-EGFP-DTA mutants exhibit PTA with stunted OFT and multiple irregular valve leaflets (indicated by * in D) and a large VSD (* in F), when compared to normal wildtype or R26-EGFP-DTA only (G) littermate control hearts.