Fig. 3.

Lipid bodies in Chrysochromulina sp. Cells were maintained on a 12 h light:12 h dark photoperiod. D11.5 cells were harvested at 11.5 h into the dark period. L11.5 cells were harvested at 11.5 h into the light. Cells were stained with BODIPY 505/515 dye (green) as described in “Materials and methods”. The chloroplast auto-fluoresces red. The scale bar is 10 μm. The cell volumes (computed from mean diameter) at these points were about 23 μm³ at D11.5 and 44 μm³ at L11.5. Excitation was at 450–490 nm and emission wavelengths were imaged through a 515 nm long-pass filter (color figure online)