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Botryococcus cells, grown in 500 mL of modified Chu-13 medium in conical Fernbach flasks upon orbital shaking. Note that (a) Botryococcus braunii var. Showa, (b) Botryococcus braunii Kawaguchi-1, (c) Botryococcus braunii Yamanaka, (d) Botryococcus braunii UTEX 2441, (e) Botryococcus braunii UTEX LB572 micro-colonies centrifuging to the center of the H2O-based growth medium, whereas (f) Botryococcus sudeticus (UTEX 2629) cultures made uniform suspension
