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. Author manuscript; available in PMC: 2011 Dec 16.
Published in final edited form as: Nature. 2010 Nov 24;468(7326):973–977. doi: 10.1038/nature09626

Figure 2. PDGFRβ upregulation is strongly correlated with PLX4032 acquired resistance.

Figure 2

a, Left, total levels of PDGFRβ and EGFR. A431, an EGFR-amplified cell line. Tubulin levels, loading control. Right, whole-cell extracts were incubated on the RTK antibody arrays, and phosphorylation status was determined by subsequent incubation with anti-phosphotyrosine horseradish peroxidase (each RTK spotted in duplicate, positive controls in corners, gene identity below). b, Anti-PDGFRβ immunohistochemistry of formalin-fixed, paraffin-embedded tissues. Prostate, negative control; placenta, positive control. Black bar, 50 μm. c, Relative RNA levels of PDGFRβ in M229 P/R5 and Pt48 R as determined by real-time, quantitative PCR (average of duplicates). d, Total PDGFRβ (left) and p-RTK (right) levels in Pt48 R versus M229 R5.