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Quantitative PCR demonstrates an increase in AAV transduction of the RPE and retina in degenerating tissue. AAV transgene copy number is significantly greater in (A) the retina and (B) RPE of TgS334ter-3 animals 5 days after intravitreal AAV5 injection (n = 10). Values represent average copy number normalized to uninjected control retina. WT, wild type.
