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. 2011 May 19;23(6):385–390. doi: 10.1093/intimm/dxr025

Fig. 6.

Fig. 6.

NFI-A-null T cells show increased c-Myc transcription that is inhibited in response to antigen receptor-mediated activation. Purified T cells from NFI-A+/+→Rag-2 or NFI-A-/→Rag-2−/− mice were stimulated with media or immobilized anti-CD3 (10 μg ml−1). Total RNA was isolated 24 h following stimulation and the expression of GRB-2, LAT, LCK, ZAP70, CD3ζ, PKB and HPRT transcripts were analyzed by semiquantitative real-time PCR as described previously (18). HPRT was used as an internal control. Panel b shows increased c-Myc transcription that is inhibited in response to antigen receptor-mediated activation in NFI-A−/−. This is in contrast to induction of c-Myc in NFI-A+/+ T cells. Results are representative of three independent experiments.