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. 2011 Jun 15;203(12):1753–1762. doi: 10.1093/infdis/jir186

Figure 4.

Figure 4.

miR-29 overexpression in LX-2 cells inhibits proliferation and collagen expression. LX-2 cells were transfected with 25 nM miR-29 mimic or negative control mimic (control-pre-miR). A, WST-1 cell proliferation assay. Average of three independent experiments in quadruplicates. B, Ki67 mRNA levels. C, Col1A1, Col3A1, and α-SMA mRNA levels. Representative experiment from three independent experiments in triplicate. Mean ± s.e.m. D, Cytotox-Fluor cytotoxicity assay. Positive control, 30 μg/ml digitonin (E) Caspase-glow 3/7 apoptosis assay. Positive control, 2 μg/ml doxorubicin. Average of three independent experiments in quadruplicate. Mean ± s.e.m. * indicates a P-value < .05 for all panels.