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. 2000 Oct 15;106(8):951–961. doi: 10.1172/JCI10905

Figure 3.

Figure 3

Vascular smooth muscle defects in the Edg1–/– embryos. (a and b) Aortae of E12.5 embryos, sectioned longitudinally and stained with anti-SMαA antibody. Note the lack of SMαA-positive smooth muscle cells (bracket) on the dorsal side of aorta in the Edg1–/– embryo. (c and d) Transverse sections of aortae from E12.5 embryos stained with anti-SMαA. Smooth muscle cells have accumulated at the ventral site of the aorta in the Edg1–/– embryo. Note the discontinuous endothelial cell (EC) layer (bracket) in the Edg1–/– embryo. Many blood cells have leaked out to the surrounding tissues in the Edg1–/– embryo (arrowheads). BC, blood cells. (e and f) H&E staining of aorta from E11.5 and 12.5 Edg1–/– embryos. Arrows point to ECs. Note their normal, flattened morphology in e and abnormal, cuboidal morphology in f. (g and h) Cranial arteries from E12.5 embryos stained with anti-SMαA antibody. Note the clustering of smooth muscle cells and nearly naked endothelial tube from the Edg1–/– embryo. (i and j) Sections of intestine from E12.5 embryos stained with anti-SMαA. Note that that coverage of intestine by smooth muscle is similar in control and mutant embryos. Scale bars = 1 mm (a and b); 50 μm (c and d); 50 μm (e and f); 50 μm (g and h); 500 μm (i and j).