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. Author manuscript; available in PMC: 2011 Sep 29.
Published in final edited form as: Cold Spring Harb Symp Quant Biol. 2011 Mar 29;75:345–356. doi: 10.1101/sqb.2010.75.030

FIGURE 1. XIST RNA paints the inactive X-chromosome (Xi) altering its chromatin composition, structure, and nuclear organization.

FIGURE 1

Note that most images show the Xi in the peripheral heterochromatic compartment of the nucleus in these human somatic cells. A) Histone H4K20 methylation, and B) histone H3K27 methylation are enriched on the Xi. C) DAPI DNA stain reveals the condensed Barr Body (arrow) at the periphery of the nucleus. D) Ubiquitinated H2A K119 is also enriched on the Xi. E) XIST RNA paints the Xi, and repeat RNA (Cot-1 RNA) is present throughout the non-nucleolar nucleoplasm, but absent from the Xi (F: arrow) and other regions of heterochromatin.